resD [2017-09-16 18:24:07]

two-component response regulator ([SW|OmpR family]), regulation of aerobic and anaerobic respiration, activates expression of target genes in response to oxygen limitation

locus: BSU23120

pI: 5.63

mw: 27.34 kDa

protein length: 240 aa

gene length: 720 bp

function: regulation of aerobic and anaerobic respiration respiration

product: two-component response regulator ([SW|OmpR family])

essential: no

synonyms: ypxD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.1|Regulators of electron transport]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 2,417,181 → 2,417,903

Phenotypes of a mutant

• a ''resD'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]

The protein

Protein family

• [SW|OmpR family] of two-component response regulators

Modification

• phosphorylated by [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 37% identity) [pubmed|17942407]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

additional information

• [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD] may interact with [SW|RNA polymerase] [pubmed|21710567]

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|22383849]

• genes: [gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|8631715]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8631715], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [PubMed|8631715,11222591], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9988472], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16825793], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]

• genes: [gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
  description: [Pubmed|8631715]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]

Biological materials

Mutant

• BKE23120 (Δ[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU23120 BGSC], upstream reverse:

  _UP1_CATGTTTTCCCCCTGCTTTC

  , downstream forward:

  _UP4_TATAAATTTGAGGTCGGCGC

• BKK23120 (Δ[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU23120 BGSC], upstream reverse:

  _UP1_CATGTTTTCCCCCTGCTTTC

  , downstream forward:

  _UP4_TATAAATTTGAGGTCGGCGC

Labs working on this gene/protein

• [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

Reviews

• 23046954

Original publications

• 8682783,15576792,16885274,16885456,11325953,10094672,17322317,8631715,9988472,10972837,10972836,9352926,16825793,8631715,11222591,9988472,21091510,22287527,21710567,24214949,27791134,26296725,28439033,17942407